DNA/RNA Amplification PCR Enzymes Fidelity PCR

Premium-Pfu Polymerase

  • Extreme fidelity and specificity
  • High processivity
  • Amplification of long-ranged target for cloning
  • PMT (Polymerase Modulator on Temperature)technology
  • Minimize non-specific amplifications and primer-dimer formation


Cat.No. Product Size
PP250 HelixAmp™ Premium-Pfu Polymerase 250 units
PP500 HelixAmp™ Premium-Pfu Polymerase 500 units
PP2500 HelixAmp™ Premium-Pfu Polymerase 2,500 units

HelixAmp™ Premium-Pfu polymerase is an editorial enzyme improving on HelixAmp™ Speed-Pfu polymerase. HelixAmp™ Premium-Pfu polymerase shows faster (4 ~ 6x) polymerization reaction and higher fidelity (3 ~ 5x) than any other high-fidelity enzyme. With this high speed and accuracy, NanoHelix’s “PMT(Polymerase Modulator on Temperature) technology” is applied in the buffer system, effectively reducing primer dimer formation and non-specific amplification during the PCR. HelixAmp™ Premium-Pfu polymerase is ideal for faithful amplification of relatively long-ranged targets for cloning etc. Due to its high speed, PCR with this enzyme can be completed in 30 min for the reliable amplification of less than 1 kb size target DNA. For the maximum performance of PCR reactions, a high-quality dNTP mixture is supplied. In case of PCR amplification of target DNA with a high G+C content or other structural problems, such as repeat sequences, the application of N-Solution™ improves the specificity and productivity of the reactions.

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