DNA/RNA Amplification Real-time PCR & RT-PCR Real-time RT-PCR

1-sec qRT-PCR Kit [Probe]

  • Fast, sensitive and reliable one-step quantitative RT-PCR kit 
  • Probe-based real-time RT-PCR kit
  • Fast real-time assay: 40 mins for 40 cycles 
  • Multiplex: Up to 5-plex probe qRT-PCR in a reaction 
  • Convenient 2x concentrated premix 
  • Unique buffer system and balanced enzyme mix


Cat.No. Product Size
SQRT-P200 RealHelix™ 1-sec qRT-PCR Kit [Probe] 200 rxns
SQRT-P500 RealHelix™ 1-sec qRT-PCR Kit [Probe] 500 rxns
The RealHelix™ 1-sec qRT-PCR kit [Probe] is designed to perform rapid real-time analysis of RNA samples using fluorescent probe based detection. This kit provides 2x premix and ROX reference dye. The convenient 2x concentrated premix contains an antibody-inhibited hot-start Taq polymerase (Ab+Taq polymerase), reverse transcriptase, RNase inhibitor, dNTPs, buffers, Mg2+, and stabilizing agent. The premix can also be used in combination with ROX reference dye in PCR instruments that are compatible with the evaluation of the ROX signal. The outstanding fast real-time assay within 40-60 min combined with high specificity and sensitivity is achieved with a unique buffer system and balanced enzyme mix.

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