qRT-PCR Kit [v4] (UDG System) is a quantitative RT-PCR reagent suitable for molecular diagnosis of SARS-CoV-2, which is causing the new Coronavirus infectious disease (COVID-19). The kit’s performance was confirmed using the materials and methods approved by WHO on 17 Jan, 2020. The enzyme mix is an optimized blend of Reverse Transcriptase, chemically-modified hot-start PCR enzyme, RNase inhibitor protein, and a thermolabile UDG. These components help ensure sensitivity, accuracy, and specificity of PCR performance for the COVID-19 molecular diagnosis. In particular, carryover contaminated PCR products can be removed by the applied UDG system.

Application 

• Quantification of target RNA by real-time RT-PCR

Figure. Multiplex qRT-PCR using the RealHelix™ qRT-PCR Kit[v4] UDG System. Purified SARS-CoV-2 viral RNA(dilution fold; 10^-4, 10^-5, 10^-6,10^-7) or human total RNA(10, 1, 0.1, 0.01ng) were analyzed using three different sets of target-specific primers and probes(ORF8 and N gene from SARS-CoV-2 / RNase P gene from human). The probes were labeled with HEX(ORF8), Texas Red(N gene), or Cy5(RNase P). The multiplex reaction was tested using the RealHelix™ qRT-PCR Kit [v4] UDG System on a real-time PCR instrument, Bio-Rad CFX96.