PureHelix™ Fast-n-Pure Plasmid Kit은 PCR, cloning, sequencing, in vitro transcription, transfection등 다양한 분자생물학적 실험에 사용할 수 있는 Plasmid DNA를 매우 빠르고 간편하게 분리할 수 있는 제품으로, 높은 binding capacity를 갖는 silica membrane을 사용하여 고순도의 plasmid DNA를 20 ㎍까지 분리할 수 있습니다. PureHelix™ Fast-n-Pure Plasmid Kit는 Alkaline lysis 방법을 기반으로 하지만, 다른 제품들과 달리 2 buffer 단계로 Plasmid DNA를 분리하기 때문에 Prep 시간이 현저히 단축되어 10분 이내에 plasmid DNA를 분리할 수 있습니다. PureHelix™ Fast-n-Pure Plasmid Kit의 Buffer S1에 포함된 pH indicator 시약은 plasmid DNA 분리 과정의 정확성을 시각적으로 확인하는데 도움을 줍니다.

 

Figure 1. An isolation of large size plasmids using PureHelix™ Fast-n-Pure Plasmid kit.

Figure 2. A purification of high-copy and low-copy plasmid DNAs using PureHelix™ Fast-n-Pure Plasmid kit.

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Figure 3. Restriction enzyme digestion of the purified plasmid using PureHelix™ Fast-n-Pure Plasmid kit. Restriction enzymatic analysis of pUC19 DNA purified using PureHelix™ Fast-n-Pure Plasmid kit 1 : Isolated uncut pUC19, 2 : Digested pUC19 with BamHI, 2 : Digested pUC19 with TaqI, 3 : Digested pUC19 with XbaI. 

Figure 4. The sequence analysis for plasmid DNA isolated by PureHelix™ Fast-n-Pure Plasmid Kit.

Application 

• Molecular biology applications 
• Automatic fluorescent sequencing 
• Restriction enzyme digestion and cloning
• Transfection of robust cells 

Quality Control

PureHelix™ Fast-n-Pure Plasmid Kit was tested for the isolation of high or low-copy plasmids and fosmid DNA from E.coli cells that contain each plasmid DNA. The high-copy plasmid DNA isolated with PureHelix™ Fast-n-Pure Plasmid Kit was tested for restriction-enzymatic digestion and the sequencing analysis.