HelixAmp™ Taq-Plus Polymerase는 HelixAmp™ Taq Polymerase의 개선 제품으로, 넓은 범위의 annealing 온도에서 target DNA를 증폭할 수 있습니다. 본 제품의 buffer에 나노헬릭스의 'PMT(Polymerase Modulator on temperature) 기술'이 적용되어 있어 primer dimer 형성 및 비특이(non-specific) 증폭을 줄이는 데 효과적입니다. 또한, 본 제품은 HelixAmp™ Taq Polymerase와 HelixAmp™ Power-Pfu Polymerase를 blending하여 최적화한 중합효소로, 일반 Taq 중합효소보다 yield과 processivity가 매우 높으며, fidelity는 일반 Taq 중합효소보다 약 4배 더 높습니다. 본 제품에 포함된 TuneUp™ Solution을 적용하면 G+C 비율이 높거나 반복 염기서열과 같은 구조적 문제가 있는 target DNA의 PCR반응에서 specificity와 productivity를 향상시킬 수 있습니다.

 

Figure 1. Comparison of HelixAmp™ Taq-Plus polymerase with other brands of Taq DNA polymerases. 12 different primer sets designed from a human genome were used in this PCR. PCR reactions were performed using 10 ng of human genomic DNA under various annealing temperatures. A: NanoHelix, B: Company S [Korea], C: Company T [Japan], and D: Company I [Korea].

 

 

 

 

 

Figure 2. Limit of detection(LOD) comparison of HelixAmpTM Taq-Plus polymerase with other companies’ related products. The indicated primer(POLD2) designed from a human genome was used in this PCR. PCR was performed using various concentrations of template. A: Company T (Japan), B: Company I (USA), C: NanoHelix (Taq polymerase), and D: NanoHelix(Taq-Plus polymerase).

 

 

 

  

Figure 3. Comparison of HelixAmp™ Taq-Plus polymerase with other companies’  related products. Different primer sets (ApoE, MD, Prion, and SBMA) designed from the indicated region of a human genome were used in this PCR. PCR was performed using 10 ng of human genomic DNA. A: Company T (Ex-Taq), B: Company T (Hot-start Taq), C: Company I (I-Taq), D: Company I (I-Star Taq), E: Company B (Top-Taq), F: Company B (Hot-start Taq), G: Company I (Platinum Taq), H: NanoHelix (Taq), and I : NanoHelix (Taq-Plus polymerase).

Storage 

• -20 ± 5 ℃

  

Shelf life 

• 12 months

  

Storage buffer 

• 20 mM Tris-HCl (pH 9.0)
• 100 mM KCl
• 0.1 mM EDTA
• 1 mM DTT
• Stabilizers
• 50 % Glycerol