RealHelix™ Superplex qPCR Kit [Probe]는 dual-labeled probe를 이용하여 multiplex qPCR을 수행 가능하도록 디자인되었습니다. 본 제품은 2배 농도의 premix와 ROX dye로 구성되어있습니다. 사용자 편의적인 2x premix에는 hot-start Taq 중합효소, heat-labile uracil-DNA glycosylase(HL-UDG), dUTP, dNTP, buffer, Mg²⁺ 및 안정제(stabilizer)와 같은 PCR에 필요한 모든 성분이 포함되어 있습니다. 적용된 Hot-start Taq 중합효소로 인하여 primer dimer와 같은 비특이(non-specific) 증폭을 최소화하고, target DNA만 특이적으로 증폭할 수 있습니다. 또한, UDG/dUTP 시스템은 이전 반응에서 발생한 PCR 산물의 carryover contamination을 방지합니다. 본 제품에 포함된 ROX는 passive reference dye로 사용할 수 있습니다.

 

Figure 1. Multiple amplification of five targets in a reaction by RealHelix™ Superplex qPCR Kit [Probe].

Five targets labeled with different fluorescent reporter dyes were analyzed with this Superplex qPCR Premix [Probe]. Reactions were performed using the RealHelix™ Superplex qPCR Kit [Probe] on a real-time PCR instrument, Bio-Rad CFX96.

 

 

 

 

Figure 2. Twelve-targets multiplex amplification in a reaction by RealHelix™ Superplex qPCR Kit [Probe].

Using the allele-specific FMMA probes (labeled with FAM, HEX, Texas Red, or Cy5), twelve rice SNP genotypes were identified in a single reaction. A mix of rice genomic DNAs (total 10 ng) was used for the FMMA reaction to detect all of the 12 SNPs screened for determination of rice variants. The multiplex reaction was performed with the RealHelix™ Superplex qPCR Kit [Probe] on a real-time PCR instrument, Bio-Rad CFX96.

Storage

• Store below -20 ℃
• ROX dye should be stored in a dark place.

 

Shelf life

• 12 months