HelixAmp™ Taq Polymerase 의 glycerol-free bulk version 입니다.

대량구매를 원하시는 경우 sale@nanohelix.net 으로 문의주시기 바랍니다.

HelixAmp™ Taq Polymerase, Glycerol-free 는 동결건조 호환이 가능하도록 고순도로 정제된 재조합효소입니다. 본 효소는 분자진단에 적용할 경우 host DNA를 거의 제거하여 위양성(false-positive) 반응을 최소화 할 수 있습니다.

별도 제공되는 10x reaction buffer에는 pH-buffering agents, salts, Mg²⁺, 및 dNTP가 포함 되어있습니다.

Application

• Routine PCR and RT-PCR
• Conventional and Real-Time PCR
• PCR for molecular diagnostics
• Manufacture of dried amplification mixtures

Enzyme activities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity

Temperature optimum: Approximately +75 ℃

Storage buffer: 20 mM Tris-HCl (pH 9.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT and stabilizers

Purity: ≥ 95 % (SDS-PAGE)

DNase contamination test: Not detectable (Incubation with 40 U enzyme and pUC19 plasmid at 37 ℃, 1 hr)

RNase contamination test: Not detectable (Incubation with 40 U enzyme and human total RNA at 37 ℃, 1 hr)

DNA contamination test: [E.coli DNA] less than 1 copy/5 U enzyme, [Human DNA] Not detectable

Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)