HelixAmp™ Premium-Taq Polymerase의 bulk version 입니다.

대량구매를 원하시는 경우 sale@nanohelix.net 으로 문의주시기 바랍니다.

HelixAmp™ Premium-Taq Polymerase 는 antibody-mediated hot start polymerase에 ㈜나노헬릭스의 PMT(Polymerase Modulator on Temperature) 기술이 적용된 효소로, 특이도(specificity)가 매우 높은 PCR 증폭이 가능합니다. 본 효소에 결합된 anti-Taq antibody는 초기 변성 단계(pre-denaturation step)에 도달할 때까지 효소 활성을 억제시키며, PMT 기술이 적용된 buffer는 PCR 과정에서 primer dimer 형성을 억제하고 비특이적 증폭을 감소시킵니다.

별도 제공되는 5x reaction buffer에는 pH-buffering agents, salts, Mg²⁺, dNTP 및 PMT additive가 포함 되어있습니다.

Application

• Antibody-mediated hot-start PCR
• Highly specific amplification while minimizing primer dimer formation
• Fast PCR assays with a short enzyme activation time and fast cycling
• Multiplex PCR
• PCR for molecular diagnostics

Figure 1. Comparison of ​HelixAmp™ Premium-Taq Polymerase with other company’s hot start versions of DNA polymerases.

12 different primer sets designed from human genome were used in this PCR. PCR was performed using 10 ng of human genomic DNA under various annealing temperatures. A : Premium-Taq [NanoHelix], B : Company I, C : Company I, D : Company T.

Figure 2. Superior performance of ​HelixAmp™ Premium-Taq Polymerase on difficult targets.

Different primer sets (ApoE, MD, Prion, COMT) designed from the indicated region of the human genome were used in this PCR. PCRs were performed using 10 ng of human genomic DNA. All of the DNA polymerases are hot-start versions of each brand. A: Company T, B: Company I, C: Company B, D: Company I, E: Company S, F: Premium-Taq [NanoHelix].

Enzyme activities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity

Enzyme activation: 2 min at +95 ℃

Storage buffer: 20 mM Tris-HCl (pH 9.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers and 50 % (v/v) glycerol

DNase contamination test: Not detectable (Incubation with 40 U enzyme and pUC19 plasmid at 37 ℃, 1 hr)

RNase contamination test: Not detectable (Incubation with 40 U enzyme and Human total RNA at 37 ℃, 1 hr)

Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)

Stability: 24 months at -20 ℃