HelixAmp™ Bst DNA Polymerase, Large Fragment 의 bulk version 입니다.

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HelixAmp™ Bst DNA Polymerase, Large Fragment 는 Bacillus stearothermophilus DNA polymerase로, DNA의 5'→3' 합성기능은 있지만, 5'→3' exonuclease 활성은 결여되어 있는 효소입니다. HelixAmp™ Bst DNA Polymerase, Large Fragment 는 E. coli 에서 발현/정제된 재조합 효소입니다.

Application

• Loop-mediated isothermal amplification (LAMP)
• DNA strand displacement amplification
• Whole genome amplification

Enzyme activities: Modified to retain 5´ → 3´ polymerase activity while lacking 5´ →3´ exonuclease activity.

Storage buffer: 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer and 50 % (v/v) glycerol

Recommended reaction temperature: 55 to 65 ℃

Purity: ≥ 95 % (SDS-PAGE)

DNase contamination test: Not detectable (Incubation with 20 U enzyme and pUC19 plasmid at 37 ℃, 1 hr)

RNase contamination test: Not detectable (Incubation with 20 U enzyme and human total RNA at 37 ℃, 1 hr)

Activity test: Correspond to reference (LAMP assay with λ phage DNA).

Stability: 24 months at -20 ℃