HelixCript™ One-Step RT-PCR Kit [Hot-Taq]는 template RNA의 one-tube 반응을 통해 높은 sensitivity와 specificity로 타켓을 증폭할 수 있습니다. Enzyme Mix에는 Thermo​ reverse transcriptase, Hot-Taq​ 중합효소 및 RNase inhibitor가 혼합되어 있습니다. 2x Reaction Mix에는 buffer, Mg²⁺ 및 dNTP가 최적화되어 혼합되어 있습니다. UDG system이 적용된 제품의 경우, 역전사(reverse transcription) 단계 전 uracil이 포함된 DNA를 효과적으로 제거하여 carryover contamination으로 인한 위양성(false positive) 결과를 방지할 수 있습니다.

 

Figure 1. Multiplex one-step RT-PCR of up to 4 targets using HelixCript™ One-Step RT-PCR Kit [Hot-Taq].

Multiplex one-step RT-PCR in a single-tube reaction was performed with total RNA extracted from a hot pepper plant (Capsicum annuum) and by adding the indicated gene-specific primer sets. Each lane from 1 to 4 represents the progressive number of primer sets. The primer sets for the following genes were used; CaAPX1 (374 bp), CaActin (461 bp), CaPF1 (615 bp), and Cadhn (761 bp).

 

Figure 2. Viral RNA detection using HelixCript™ One-Step RT-PCR Kit [Hot-Taq].

Total RNA was isolated from a nasopharyngeal specimen of a new influenza virus-infected patient. Each reaction was performed with one of these target-specific primer sets: influenza A(InfA, 106 bp), Swine influenza A (SW InfA, 195 bp), Swine Influenza H1(SW H1,116 bp), or RNase P(human internal control, 65 bp).

Storage

• Store below -20 ℃

 

Shelf life

• 12 months