HelixAmp™ Genome Amplifier(WGA)는 아주 적은 양의 genome DNA로부터 whole genome을 증폭할 수 있습니다. Phi29 DNA 중합효소를 이용한 등온성 multiple displacement amplification(isothermal MDA)을 통해 genome이 증폭됩니다. Strand displacement와 강력한 3'→5' exonuclease 및 extreme processivity 활성으로 높은 fidelity를 갖는 Phi29 DNA 중합효소에 의해 최대 100kb 까지 DNA를 합성할 수 있습니다. 본 제품을 사용하면 90분 이내에 10ng genomic DNA에서 약 1,000배 증폭된 DNA를 얻을 수 있습니다. 증폭된 genome은 PCR, genotyping, library construction과 같은 다운스트림 유전자 분석(downstream genetic analysis) 실험에 바로 사용할 수 있습니다.

 

Figure 1. WGA reactions from 10 ng of human genomic DNA using HelixAmp™Genome Amplifier. Reactions were performed in 20 ㎕ volume at 30℃ for 1.5 hours. 3 ㎕ of each reaction solution was loaded on a 1% agarose gel for analysis.  

 

 

 

Figure 2.  Time-course measurements of DNA amplification using HelixAmp™ Genome Amplifier from 10 ng of genomic DNA purified from blood collections. Ten ng of Genomic DNA samples were amplified using the HelixAmp™Genome Amplifier, and the DNA yields of each reaction were determined by reaction time. About 8 ul of products were obtained in 60 minutes and 12 ul after 90 min incubations.

 

 

 

 

 

Figure 3. Validation of amplified genome products by chromosome-targeting PCR analysis. Ten ng of human genomic DNA, isolated from blood collection, was applied in the whole genome amplification using HelixAmp™Genome Amplifier. (A) PCR detections of human beta-actin gene from the amplified genomes. Purification is not required for the PCRs using the products of HelixAmp™Genome Amplifier.(B) PCR detections of each chromosome-specific region of chromosome 7, 8, 10, and 17 were performed using the amplified whole genome as the template. The amounts of the template used in each 50 ㎕ PCR reactions are: lane 1, 1 ㎕; lane 2; 0.1 ㎕, lane 3; 0.01 ㎕ of the amplified genome product and lane 4; 1 ng of the purified human genomic DNA as a control.

Storage

• -20 ± 5 ℃

Shelf life

• 6 months