PureHelix™ Genomic DNA Prep Kit (Ver. 3.0), Column type is designed for rapid and pure isolation of total DNA from whole blood, cultured animal cells, bacteria [Gram (+) or Gram (-)], animal tissues, plant tissues, rice, or yeast cells. The spin column based method completely removes PCR inhibitors such as divalent cations and proteins resulting in a high purity preparation of genomic DNA. There is no use of phenol or chloroform, handling is safe and does not produce any harmful waste. DNA purified with this kit is suitable for a variety of applications, including PCR amplification, digestion with restriction endonucleases and membrane hybridizations.

 

 

Figure 1. Isolation of genomic DNA from various samples using PureHelix™ Genomic DNA Prep Kit (Ver. 3.0), Column type.

The isolation of genomic DNA from 500 μl of liquid-cultured E.coli (2 x 10⁹ cell/ml) and pepper leaf tissue, cow muscle, and human blood. Purification of genomic DNAs was performed following the manufacturer’s manual.

 

 

 

 

 

Figure 2. Typical yields of pure genomic DNA obtained from various animal or plant samples.

N: Genomic DNA purified by PureHelix™; Q: by DNeasy®. The isolation of genomic DNA from 80 mg of various tissues. Purification of genomic DNAs was performed following the manufacturer’s manual. A: Animal tissues, B: Plant tissues, Gm: Glycine max (soybean), Os: Oryza sativa (rice), Nt: Nicotiana tabacum, Oc: Oriental cherry, Ca: Capsicum annuum (pepper), Sl: Solanum lycopersicum (tomato).

Storage

• Room temperature
  

 

Shelf life

• 12 months