PureHelix™ PCR Purification Kit is designed for rapid and high-yield clean-up DNA from PCR and other enzymatic reaction mixtures. PureHelix™ PCR Purification Kit contains a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, proteins, salts and other impurities from DNA samples. The high purity DNA isolated using this kit is adequate for any molecular biology applications. Especially, the purification of PCR products using the dimer removal condition for an efficient removal of primer dimers, by-products of PCR, ensures excellent results of the automating sequence analysis.

 

 

Figure 1. The PCR purification of PCR products of various sizes using PureHelix™ PCR Purification Kit.

C : PCR products before purification, Q : PCR products purified by QIAquick PCR Purification Kit. Each PCR product is finally eluted with a 50 μl elution buffer, and 10 % volume of each sample is electrophoresed in an agarose gel. 

 

 

 

 

 

Figure 2. The PCR purification under ‘high yield method’.

C: PCR products (100 bp) before purification; Q: PCR product purified by QIAquick PCR Purification Kit. Each PCR product was eluted with 50 μl of elution buffer. 

 

 

 

 

 

Figure 3. PCR purification under ‘dimer removal method’.

B: PCR product mixture (100, 300, and 600 bp) before purification; D: PCR product purified under ‘dimer removal method’; H: PCR product purified under ‘high yield method’. 

 

 

 

 

 

Figure 4. Accurate sequencing of a purified 1kb PCR product.

(A), O: PCR product derived from lambda DNA before purification, P: PCR product purified by PCR purification kit under ‘dimer removal method’. (B) Sequencing result

Storage

• Room temperature
  

 

Shelf life

• 12 months