HelixAmp™ Taq Polymerase is a recombinant enzyme expressed and purified from a bacterial host cell harboring Thermus aquaticus DNA polymerase gene. HelixAmp™ Taq Polymerase is an engineered Taq DNA polymerase enforced its thermostability and template sensitivity. This highly purified thermostable DNA polymerase with unique NanoHelix’s purification process is quite suitable for routine PCR. For the maximum performance extremely pure dNTPs and TuneUp™ Solutions are also included. TuneUp™ Solution helps HelixAmp™ Taq Polymerase to efficiently amplify the problematic target region of high G+C content or structural problem.

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Figure 1. The purity of HelixAmp™ Taq Polymerase.

The purity and concentration of the prepared enzyme were analyzed by SDS-PAGE with each unit represented. St: Standard Protein marker.

Figure 2. The sensitivity of HelixAmp™ Taq Polymerase.

HelixAmp™ Taq Polymerase amplified targets from the genomic region of human PKD (polycystic kidney disease) gene at various concentrations of human genomic DNA. 1.25 U of Taq DNA polymerases manufactured by NanoHelix and other companies were used.

Figure 3. Comparison of the PCR performances of commercial Taq polymerases on various sizes of targets.

The PCRs were performed with 1.25 units of Taq polymerases and supplied buffers from each manufacturer. All other components and conditions applied are identical throughout the reactions.

Figure 4. Superior activity of HelixAmp™ Taq Polymerase.

The PCRs were performed with indicated units of Taq polymerases and supplied buffers from each manufacturer. All other components and conditions applied are identical throughout the reactions. NC, negative control reactions were performed with 2.5 units of enzymes without template DNA.