Products

DNA-RNA Amplification PCR Enzymes Specific PCR

Hot-Taq Polymerase (Ver.2.0)

  • Chemically modified Taq DNA polymerase
  • Automatic hot-start PCR enzyme
  • Convenience of reactions set up at room temperature
  • The highest specificity of PCR amplification
  • Enzyme for real-time PCR and multiplex PCR
  • Enzyme for high [GC] target amplification

Products

Cat.No. Product Size
HT250 HelixAmp™ Hot-Taq Polymerase (Ver. 2.0) 250 units
HT500 HelixAmp™ Hot-Taq Polymerase (Ver. 2.0) 500 units
HT2500 HelixAmp™ Hot-Taq Polymerase (Ver. 2.0) 2,500 units
HT250N HelixAmp™ Hot-Taq Polymerase (with dNTP) (Ver. 2.0) 250 units
HT500N HelixAmp™ Hot-Taq Polymerase (with dNTP) (Ver. 2.0) 500 units
HT2500N HelixAmp™ Hot-Taq Polymerase (with dNTP) (Ver. 2.0) 2,500 units
HTBF250 HelixAmp™ Hot-Taq Polymerase [MgCl₂ free] (Ver. 2.0) 250 units
HTBF500 HelixAmp™ Hot-Taq Polymerase [MgCl₂ free] (Ver. 2.0) 500 units
HTBF2500 HelixAmp™ Hot-Taq Polymerase [MgCl₂ free] (Ver. 2.0) 2,500 units
HTBF250N HelixAmp™ Hot-Taq Polymerase [MgCl₂ free] (with dNTP) (Ver. 2.0) 250 units
HTBF500N HelixAmp™ Hot-Taq Polymerase [MgCl₂ free] (with dNTP) (Ver. 2.0) 500 units
HTBF2500N HelixAmp™ Hot-Taq Polymerase [MgCl₂ free] (with dNTP) (Ver. 2.0) 2,500 units

HelixAmp™ Hot-Taq Polymerase (Ver. 2.0) is a chemically-modified form of purified Taq DNA polymerase and suitable for high-specificity hot-start PCR, real-time PCR and multiplex PCR. The attached heat-labile chemical moiety makes Taq DNA polymerase inactive and suppresses the polymerization from non-specifically bound primers which occurs during the setting of PCR mix and first ramp-up of thermal cycling. During the first denaturation step of PCR the chemical moieties are released from Taq polymerase and the enzyme turns to be active. With the high specificity, HelixAmp™ Hot-Taq Polymerase (Ver. 2.0) shows high performance in the reactions of genotyping (microsatellite or SNP), multiplex PCR, and real-time PCR. For the maximum performance, extremely pure dNTPs and TuneUp™ Solutions are also included. TuneUp™ Solution helps the DNA polymerase to efficiently amplify the problematic target region of high G+C content or structural problem.

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