HelixAmp™ Hot-Taq Polymerase (Ver. 2.0) is a chemically-modified form of purified Taq DNA polymerase and suitable for high-specificity hot-start PCR, real-time PCR and multiplex PCR. The attached heat-labile chemical moiety makes Taq DNA polymerase inactive and suppresses the polymerization from non-specifically bound primers which occurs during the setting of PCR mix and first ramp-up of thermal cycling. During the first denaturation step of PCR the chemical moieties are released from Taq polymerase and the enzyme turns to be active. With the high specificity, HelixAmp™ Hot-Taq Polymerase (Ver. 2.0) shows high performance in the reactions of genotyping (microsatellite or SNP), multiplex PCR, and real-time PCR. For the maximum performance, extremely pure dNTPs and TuneUp™ Solutions are also included. TuneUp™ Solution helps the DNA polymerase to efficiently amplify the problematic target region of high G+C content or structural problem.

Concentration

• Hot-Taq Polymerase (Ver. 2.0) : 2.5 units/μl

Storage

• Store below -20 ℃

Shelf life

• 24 months

Storage buffer

• 20 mM Tris-HCl (pH 9.0)
• 100 mM KCl
• 0.1 mM EDTA
• 1 mM DTT
• Stabilizers
• 50 % Glycerol