HelixAmp™ DNA-free Hot-Taq Polymerase is a recombinant enzyme expressed and purified from a bacteria host cell harboring Thermus aquaticus DNA polymerase gene and purified using a process that minimizes level of contaminating host DNA. HelixAmp™ DNA-free Hot-Taq Polymerase is a chemically-modified form of purified DNA-free Taq DNA polymerase and quite suitable for high-specific hot-start PCR, real-time PCR and multiplex PCR. Because the contaminating host genomic DNA can cause the false positive result, use of DNA-free Hot-Taq polymerase is very important in PCR detection of bacteria with universal primers (e.g. Primers targeting 16s rRNA gene).

Figure 1. Contamination test of host DNA in HelixAmp™ DNA-free Hot-Taq Polymerase.

Contamination test of host DNA in conventional Taq DNA polymerase HelixAmp™ Hot-Taq Polymerase (Ver. 2.0) and HelixAmp™ DNA-free Hot-Taq Polymerase using a real-time PCR assay.

Figure 2. Comparison of the contamination of host DNA.

Comparison of conventional Taq DNA polymerase and HelixAmp™ DNA-free Taq Polymerase for the contamination of host DNA. PCRs were performed with or without the template. Primers are specific for the E. coli 16s rRNA-coding gene.

Concentration

• DNA-free Hot-Taq Polymerase : 2.5 units/μl

Storage

• Store below -20 ℃

Shelf life

• 24 months

Storage buffer

• 20 mM Tris-HCl (pH 9.0)
• 100 mM KCl
• 0.1 mM EDTA
• 1 mM DTT
• Stabilizers
• 50 % Glycerol