HelixAmp™ Power-Pfu Polymerase, originated from an extreme thermophile, is ideal for high-fidelity PCR amplification. Due to its intrinsic 3’ to 5’ exonuclease (proof-reading) activity, this DNA polymerase shows high-fidelity and produces blunt ended PCR products. NanoHelix’s “PMT (polymerase modulator on temperature) technology” is applied in the buffer system, which is effective to reduce primer-dimer formation and non-specific amplification during the PCR. HelixAmp™ Power-Pfu Polymerase is an engineered enzyme showing much higher productivity and processivity than other proof-reading thermostable enzymes (e.g. Pfu DNA polymerase, Vent DNA polymerase etc.) without compromise to the fidelity. For the maximum performance of PCR reactions high-quality dNTP mixture is supplied. The addition of TuneUp™ Solution in the reaction mixture is a simple way to optimize the difficult targets of PCR.

Concentration

• Power-Pfu Polymerase : 2.5 units/μl

 

Storage

• -20 ± 5 ℃

 

Shelf life

• 24 months

 

Storage buffer

• 50 mM Tris-HCl (pH 8.0)
• 0.1 mM EDTA
  
• 1 mM DTT
• Stabilizers
• 50 % Glycerol