HelixAmp™ Power-Pfu Polymerase, originated from an extreme thermophile, is ideal for high-fidelity PCR amplification. Due to its intrinsic 3’ to 5’ exonuclease (proof-reading) activity, this DNA polymerase shows high-fidelity and produces blunt ended PCR products. NanoHelix’s “PMT (polymerase modulator on temperature) technology” is applied in the buffer system, which is effective to reduce primer-dimer formation and non-specific amplification during the PCR. HelixAmp™ Power-Pfu Polymerase is an engineered enzyme showing much higher productivity and processivity than other proof-reading thermostable enzymes (e.g. Pfu DNA polymerase, Vent DNA polymerase etc.) without compromise to the fidelity. For the maximum performance of PCR reactions high-quality dNTP mixture is supplied. The addition of TuneUp™ Solution in the reaction mixture is a simple way to optimize the difficult targets of PCR.

 

 

 

Figure 1. Various annealing temperatures of HelixAmp™ Power-Pfu Polymerase. 12 different primer sets designed from the human genome were used in this PCR. PCR was performed using 10 ng of human genomic DNA under the various annealing temperatures. AT = annealing temperature.

 

 

 

 

 

Figure 2. The fidelities of PCR enzymes. The fidelity of HelixAmp™ Power-Pfu Polymerase was compared with high fidelity enzymes from other companies and Taq polymerase. Error rate = mutation/bp/duplication.

Concentration

• Power-Pfu Polymerase : 2.5 units/μl

 

Storage

• -20 ± 5 ℃

 

Shelf life

• 24 months

 

Storage buffer

• 50 mM Tris-HCl (pH 8.0)
• 0.1 mM EDTA
  
• 1 mM DTT
• Stabilizers
• 50 % Glycerol