HelixAmp™ Premium-Pfu Polymerase is an editorial enzyme improved from HelixAmp™ Speed-Pfu Polymerase. HelixAmp™ Premium-Pfu Polymerase shows faster (4 ~ 6 times) polymerization reaction and higher fidelity (3 ~ 5 times) than any other high-fidelity enzymes. NanoHelix’s “PMT (polymerase modulator on temperature) technology” is applied in the buffer system, which is effective to reduce primer-dimer formation and non-specific amplification during the PCR. HelixAmp™ Premium-Pfu Polymerase is most suitable to faithful amplification of relatively long-ranged target for cloning etc. Due to its high speed, fast PCR with this enzyme could be completed in 30 min for the reliable amplification of less than 1 kb size target DNA. For the maximum performance of PCR reactions high-quality dNTP mixture is supplied. In case of PCR amplification of target DNA with high G+C content or structural problem, such as repeat sequence, the application of N-Solution™ improves the specificity and productivity of the reactions.

Concentration

• Premium-Pfu Polymerase : 2.5 units/μl

 

Storage

• -20 ± 5 ℃

 

Shelf life

• 12 months

 

Storage buffer

• 50 mM Tris-HCl (pH 8.0)
• 0.1 mM EDTA
  
• 1 mM DTT
• Stabilizers
• 50 % Glycerol