HelixAmp™ Speed-Pfu Polymerase is an editorial enzyme improved from HelixAmp™ Power-Pfu Polymerase. HelixAmp™ Speed-Pfu Polymerase shows faster (4 ~ 6 times) polymerization reaction and higher fidelity (3 ~ 5 times) than any other high-fidelity enzymes. With this high speed and accuracy, HelixAmp™ Speed-Pfu Polymerase is most suitable to faithful amplification of relatively long-ranged target for cloning etc. Due to its high speed, fast PCR with this enzyme could be completed in 30 min for the reliable amplification of less than 1 kb size target DNA. For the maximum performance of PCR reactions high-quality dNTP mixture is supplied. The addition of TuneUp™ Solution in the reaction mixture is a simple way to optimize the difficult targets of PCR.

 

 

 

Figure 1. The express speed of HelixAmp™ Speed-Pfu Polymerase. Four different sizes of human gene fragments were amplified with various extension times. Four PCR reactions were carried out in a two-step thermocycling condition (30 cycles of 95 ℃ for 10 sec, 68 ℃ for 20, 40, 60, or 90 sec respectively). This result indicates that only 20-23 seconds is required for successful synthesis of 1 kb size DNA. The total PCR time required for amplification of 1 kb fragment is less than 25 minutes. 

 

 

 

 

 

Figure 2. A fast PCR with HelixAmp™ Speed-Pfu Polymerase and other enzymes. 1 kb fragment of human DNA was amplified from various concentrations of template in a limited extension time, 20 sec. PCR reaction was carried out at 95 ℃/10 sec, and 68 ℃/20 sec (35 cycles). 

 

 

 

 

 

Figure 3. HelixAmp™ Speed-Pfu Polymerase is the enzyme with the highest fidelity. Error Rate = mutation/bp/duplication

Concentration

• Speed-Pfu Polymerase : 2.5 units/μl

 

Storage

• -20 ± 5 ℃

 

Shelf life

• 24 months

 

Storage buffer

• 50 mM Tris-HCl (pH 8.0)
• 0.1 mM EDTA
  
• 1 mM DTT
• Stabilizers
• 50 % Glycerol