RealHelix™ qPCR Kit [Probe] is designed to perform a rapid real-time quantification of target DNA using dual-labeled probes. The convenient 2x concentrated premix contains hot-start PCR enzyme, dNTPs, buffers, Mg²⁺, and stabilizing agent. The hot-start PCR enzyme provides high specific amplification of target DNA and minimizes the non-specific reactions and production of primer dimers. The separately supplied ROX dye could be used as a passive reference dye in real-time PCR instruments that are compatible with the evaluation of the ROX signal.

Figure 1. The accuracy of real-time PCR using RealHelix™ qPCR Kit [Probe].

Human genomic DNAs (10, 1, 0.1, and 0.01 ng) isolated from whole blood were analyzed using the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene-specific primer set and 5’-FAM-labelled probe. The accuracy of real-time PCR is demonstrated by E=99.9 %, R² value=0.997. Performed on a CFX96 real-time PCR detection system (Bio-Rad).

Figure 2. Comparison of real-time PCR using RealHelix™ qPCR Kit [Probe] and its previous version (qPCR Kit [Probe type]).

Human genomic DNAs (100, 10, 1, and 0.1 ng) isolated from whole blood were analyzed using the beta-globin gene-specific primer set and 5’-Quasar705-labelled probe. Performed on a CFX96 real-time PCR detection system (Bio-Rad).

Storage

• Store below -20 ℃
• ROX dye should be stored in a dark place.

Shelf life

• 12 months