Raw Materials for MDx PCR Enzymes for MDx
Premium-Taq Polymerase
5 units/μl
- Minimize the non-specific amplification by a dual control system;
- Anti-Taq antibody complexed Taq DNA polymerase
- PMT technology applied buffer system - Highly specific and sensitive PCR enzyme
- Automatic hot-start PCR enzyme
- Fast enzyme activation
Products
Cat.No. | Product | Feature | Size |
---|---|---|---|
BPT5 | HelixAmp™ Premium-Taq Polymerase (5unit/μl), Bulk | 5 units/μl | Custom |
This is the bulk version of HelixAmp™ Premium-Taq Polymerase.
If you would like to purchase in bulk, please contact sale@nanohelix.net.
HelixAmp™ Premium-Taq Polymerase is an anti-Taq antibody complex form of Taq polymerase and ideal for automatic hot-start PCR. The inhibitory anti-Taq antibody inactivates the polymerase by binding at low temperature and suppresses the polymerization from non-specifically bound primers which occurs during the setting of PCR mix and the temperature going up in the PCR machine. At the high temperature of the initial denaturation step of PCR, the antibody is released by denaturation and the free Taq DNA polymerase becomes active. Unlike chemically modified hot-start Taq polymerase, this enzyme needs not prolonged (10 to 15 min.) incubation at above 94℃ to be active. “NanoHelix’s PMT (Polymerase Modulator on Temperature) technology” is applied in the buffer system, which is effective to reduce primer-dimer formation and non-specific amplification during the PCR. The 5x Reaction Mix [PT] contains buffers, dNTPs, Mg2+, and glycerol in appropriate concentrations. In case of PCR amplification of target DNA with high G+C content or structural problem, such as repeat sequence, N-Solution™ will improves the specificity and productivity of the reactions.
Enzyme only; 5x Reaction Mix and other components are available separately upon request.
Application
- Hot-Start PCR
- Real-Time PCR
- Genotyping
- Multiplex PCR