This is the bulk version of HelixAmp™ Premium-Taq Polymerase.

If you would like to purchase in bulk, please contact sale@nanohelix.net.

​HelixAmp™ Premium-Taq Polymerase provides the most specific amplification using a dual system of antibody-mediated hot start polymerase and PMT (Polymerase Modulator on Temperature)-applied buffer. Anti-Taq antibody inhibits the Taq polymerase activity until it reaches denaturation temperature while the PMT-applied buffer reduces the primer-dimer formation and non-specific amplification during the PCR cycles. The separately available 5x reaction buffer contains pH-buffering agents, salts, Mg²⁺, dNTPs and PMT additive.

Application

• Antibody-mediated hot-start PCR
• Highly specific amplification while minimizing primer dimer formation
• Fast PCR assays with a short enzyme activation time and fast cycling
• Multiplex PCR
• PCR for molecular diagnostics

Figure 1. Comparison of ​HelixAmp™ Premium-Taq Polymerase with other company’s hot start versions of DNA polymerases.

12 different primer sets designed from human genome were used in this PCR. PCR was performed using 10 ng of human genomic DNA under various annealing temperatures. A : Premium-Taq [NanoHelix], B : Company I, C : Company I, D : Company T.

Figure 2. Superior performance of ​HelixAmp™ Premium-Taq Polymerase on difficult targets.

Different primer sets (ApoE, MD, Prion, COMT) designed from the indicated region of the human genome were used in this PCR. PCRs were performed using 10 ng of human genomic DNA. All of the DNA polymerases are hot-start versions of each brand. A: Company T, B: Company I, C: Company B, D: Company I, E: Company S, F: Premium-Taq [NanoHelix].

Enzyme activities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity

Enzyme activation: 2 min at +95 ℃

Storage buffer: 20 mM Tris-HCl (pH 9.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers and 50 % (v/v) glycerol

DNase contamination test: Not detectable (Incubation with 40 U enzyme and pUC19 plasmid at 37 ℃, 1 hr)

RNase contamination test: Not detectable (Incubation with 40 U enzyme and human total RNA at 37 ℃, 1 hr)

Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)

Stability: 24 months at -20 ℃