This is the bulk version of HelixCript™ One-Step RT-PCR Kit [Hot-Taq].

If you would like to purchase in bulk, please contact sale@nanohelix.net.

HelixCript™ One-Step RT-PCR Kit [Hot-Taq] is designed for specific and sensitive amplification of target genes in one-tube reactions from RNA templates. Thermo​ Reverse Transcriptase, Hot-Taq​ polymerase, and RNase Inhibitor comprise the enzyme mixture. An optimized buffer is provided as a 2x Reaction mix containing a buffer, MgCl₂, and dNTPs. Optional UDG system includes the Heat-Labile UDG and dUTP.

Application

• Viral RNA detection
• Gene-expression analysis
• Molecular diagnosis
• Endpoint one-step RT-PCR from any RNA transcript

Figure 1. Multiplex one-step RT-PCR of up to 4 targets using HelixCript™ One-Step RT-PCR Kit [Hot-Taq].

Multiplex one-step RT-PCR in a single-tube reaction was performed with total RNA extracted from a hot pepper plant (Capsicum annuum) and by adding the indicated gene-specific primer sets. Each lane from 1 to 4 represents the progressive number of primer sets. The primer sets for the following genes were used; CaAPX1 (374 bp), CaActin (461 bp), CaPF1 (615 bp), and Cadhn (761 bp).

Properties: Conventional RT-PCR, Hot-start

Heat activation: +95 ℃ for 15 minutes after reverse transcription reaction

Activity test: Performance test in PCR (Human total RNA, Actin fragment): Corresponds to specification

Stability: 12 months at -20 ℃