This is the bulk version of RealHelix™ Direct qPCR Kit [Probe].

If you would like to purchase in bulk, please contact sale@nanohelix.net.

RealHelix™ Direct qPCR Kit [Probe] is designed for a probe-based qPCR amplification directly from animal tissues, plant tissues, and various clinical samples including whole blood, serum, urine, hair and swab collections without any DNA purification processes. The 2x Direct qPCR Premix [Probe] in this kit contains antibody-inhibited Taq DNA polymerase, dNTPs, MgCl₂, stabilizer, and unique buffer system to resist various PCR inhibitors of tissue samples.

Figure. Real-time PCR using RealHelix™ Direct qPCR Kit [Probe] from various human samples.

The lysates from each sample (whole blood, buccal swab, or urine) were prepared following the protocol. Each 1 μl of lysate and 10 ng of human genomic DNA (positive control) were analyzed using a human beta-globin gene-specific primer set and a probe labeled with FAM on a Real-Time PCR instrument, Bio-Rad CFX96. NTC, no template control. Each reaction was duplicated.

Storage

• Store below -20 ℃

Shelf life

• 12 months