This is the bulk version of RealHelix™ qPCR Kit [Probe].

If you would like to purchase in bulk, please contact sale@nanohelix.net.

RealHelix™ 2x qPCR Premix [Probe] is designed to perform a rapid real-time quantification of target DNA using dual-labeled probes. The convenient 2x concentrated premix contains hot-start PCR enzyme, dNTPs, buffers, Mg²⁺, and stabilizing agent. The hot-start PCR enzyme provides high specific amplification of target DNA and minimizes the non-specific reactions and production of primer dimers. The separately supplied ROX dye could be used as a passive reference dye in real-time PCR instruments that are compatible with the evaluation of the ROX signal. Bulk version is premix only. ROX reference dye is available separately upon request.

Figure 1. The accuracy of real-time PCR using RealHelix™ qPCR Kit [Probe].

Human genomic DNAs (10, 1, 0.1, and 0.01 ng) isolated from whole blood were analyzed using the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene-specific primer set and 5’-FAM-labelled probe. The accuracy of real-time PCR is demonstrated by E=99.9 %, R² value=0.997. Performed on a CFX96 real-time PCR detection system (Bio-Rad).

Figure 2. Comparison of real-time PCR using RealHelix™ qPCR Kit [Probe] and its previous version (qPCR Kit [Probe type]).

Human genomic DNAs (100, 10, 1, and 0.1 ng) isolated from whole blood were analyzed using the beta-globin gene-specific primer set and 5’-Quasar705-labelled probe. Performed on a CFX96 real-time PCR detection system (Bio-Rad).

Storage

• Store below -20 ℃
• ROX dye should be stored in a dark place.

Shelf life

• 12 months