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RealHelix™ qRT-PCR Kit [v4] [UDG System] is a quantitative RT-PCR reagent that efficiently facilitates reproducible, reliable multiplex detection of up to five RNA targets in a single tube reaction. The enzyme mix is an optimized blend of reverse transcriptase, chemically-modified hot-start PCR enzyme, RNase inhibitor protein, and a thermolabile UDG. The 2x reaction buffer contains all required components, including Mg²⁺, dUTP, and dNTPs. These components help ensure sensitivity, accuracy, and specificity of PCR performance. In addition, carryover-contaminated PCR products can be removed by the applied UDG system.

Figure. Multiplex qRT-PCR using the RealHelix™ qRT-PCR Kit [v4] [UDG System].

Purified SARS-CoV-2 viral RNA(dilution fold; 10^-4, 10^-5, 10^-6, 10^-7) or human total RNA(10, 1, 0.1, 0.01ng) were analyzed using three different sets of target-specific primers and probes(ORF8 and N gene from SARS-CoV-2 / RNase P gene from human). The probes were labeled with HEX(ORF8), Texas Red(N gene), or Cy5(RNase P). The multiplex reaction was tested using the RealHelix™ qRT-PCR Kit [v4] [UDG System] on a real-time PCR instrument, Bio-Rad CFX96.

Storage

• Store below -20 ℃

Shelf life

• 12 months