This is the bulk version of HelixAmp™ Phi29 DNA Polymerase.

If you would like to purchase in bulk, please contact sale@nanohelix.net.

 

HelixAmp™ Phi29 DNA Polymerase is a recombinant protein purified from E.coli cloned the gene encoding the DNA polymerase from phi29 phage. Phi29 DNA polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 and possesses the highest processivity and strand-displacement activity among the known DNA polymerase. Phi29 DNA polymerase contains a 3´→5´ exonuclease activity that enables proofreading capability.

 

 

Application

• Highly accurate DNA synthesis
• Rolling circle amplification (RCA)
• Multiple displacement amplification (MDA)
• Amplification of whole genome (WGA)

Enzyme activities: Highly processive polymerase featuring strong strand displacement activity which allows for highly efficient isothermal DNA amplification.

 

Storage buffer: 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizers and 50 % (v/v) glycerol

 

Purity: ≥ 90 % (SDS-PAGE)

 

Activity test: Correspond to reference (WGA assay with human and plant genomic DNA)

 

Stability: 12 months at -20 ℃