HelixAmp™ Fast RT-LAMP Lyo-Cake (V3), a lyophilized RT-LAMP reagent, provides a one-step solution for loop-mediated isothermal amplification (LAMP) of RNA targets. Especially this kit suppress non- specific product formation in isothermal amplification. Through the lyophilization method, moisture is removed from the product to facilitate storing or transporting products at room temperature. The Lyophilized RT-LAMP premix can be rehydrated simply by adding the Rehydration Buffer along with primers and templates.
HelixAmp™ Fast RT-LAMP Lyo-Cake(V3), contains engineered Bst DNA Polymerase, thermo-stable reverse transcriptase(RTase), RNase inhibitor, dNTPs, Mg²⁺, salts, and stabilizing agents. This kit allows the fast amplification of the target within 30minutes with an appropriate LAMP primer set. The thermo-stable RTase is fully active at a relatively high temperature (60℃), making the one-step RT-LAMP possible at a constant reaction temperature.