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Heat Labile UDG

  • Removes uracil-containing DNA
  • Optimum temperature: 15 ℃ ~ 25 ℃
  • Heat-labile, inactivated at over 50 ℃
  • Apply to a one-step RT-PCR to prevent carryover contamination

Products

Cat.No. Product Size
HLU200 HelixZyme™ Heat Labile UDG 200 units
HLU500 HelixZyme™ Heat Labile UDG 500 units
HelixZyme™ Heat Labile UDG is a recombinant uracil-DNA glycosylase from marine bacterium BMTU 3346. UDG catalyzes the hydrolysis of the N-glycosidic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. HelixZyme™ Heat Labile UDG is fully active in the temperature range of 15 to 25 ℃ and inactivated at over 50 ℃. Due to this character, this enzyme is ideal for applying on the one-step RT-PCR system to remove the contaminated uracil-containing DNA in the reaction.

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