DNA-RNA Amplification Direct Amplification

DirectFast qPCR Kit

  • Direct: DNA extraction-free
  • Fast(< 30 minutes) or ultrafast(< 20 minutes) for 40 cycles
  • Multiplex: Up to 5-plex probes in a reaction
  • Ab-based hot start Taq polymerase / UDG system


Cat.No. Product Size
DFQPU-T100 RealHelix™ DirectFast qPCR Kit 100 rxns

RealHelix™ DirectFast qPCR Kit is designed for a probe-based rapid qPCR amplification directly from animal tissues, plant tissues, and various clinical samples (including whole blood, serum, urine, hair and swab collections) without any DNA purification processes. The 2x DirectFast qPCR Premix contains antibody-inhibited Taq DNA polymerase, thermo-labile uracil-DNA glycosylase, dUTP, dNTPs, MgCl₂, a stabilizer, and a unique buffer system to resist various PCR inhibitors of tissue samples. The applied UDG/dUTP system prevents the carryover contamination of PCR products from previous reactions.

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