DNA-RNA Amplification Direct Amplification
Direct WGA Kit
- Fast and uniform amplification across entire genome
- Multiple displacement amplification by Phi29 DNA polymerase
- Direct WGA from whole blood, animal tissues, plant leaves and seeds, and clinical & forensic sample
Products
| Cat.No. | Product | Size |
|---|---|---|
| DWGA50 | HelixAmp™ Direct WGA Kit | 50 rxns |
| DWGA100 | HelixAmp™ Direct WGA Kit | 100 rxns |
HelixAmp™ Direct WGA Kit is a complete system for whole genome amplification from various tissues or
samples directly without DNA purification processes. Very little amount of samples, several milligram or
microliter volume, are required for the direct WGA. About 10 μg DNA products could be obtained in a standard
reaction. The enzyme mix and buffer system are designed to tolerate against most amplification inhibitors
found in crude samples. Phi29 DNA polymerase, the major polymerization enzyme of this kit, isothermally
amplifies the genomic DNAs included in the samples with multiple displacement mechanism. Phi29 DNA
polymerase could produce DNA strand up to 70 kb long with high fidelity. All required components including
enzymes, buffers, dNTPs, random primers, and sample pretreatment reagents are supplied in this kit. The
amplified DNA products could be applied for successive PCR, genotyping, and library construction.
