HelixCript™ One-Step RT-PCR Kit [Speed-Pfu] is designed for sensitive amplification of the target gene in one-tube reaction from total transcripts. A reverse transcriptase, HelixCript™ Thermo Reverse Transcriptase, and a thermostable DNA polymerase, HelixAmp™ Speed-Pfu Polymerase are supplied as an enzyme mixture. 

Figure 1. A fast RT-PCR using HelixCript™ One-Step RT-PCR Kit [Speed-Pfu].

One-Step RT-PCR reactions were performed under the modified protocol as follows : The total time required for a One-Step RT-PCR reaction is 1 hour. One-step RT-PCRs were performed with 1 to 100 ng of human blood total RNA using HelixCript™ One-Step RT-PCR Kit [Speed-Pfu] and the primer set for human β-globin. The PCR reaction was carried out at 95 ℃ (10 sec), 55 ℃ (10 sec), and 72 ℃ (20 sec) for 35 cycles.

Storage

• -20 ± 5 ℃

Shelf life

• 12 months