DNA-RNA Amplification Isothermal Amplification
Fast RT-LAMP Kit (Ver.2.0)
- Fast detection (<20 minutes) for RNA target
- Sensitive and accurate
- Flexible detection method: Endpoint and real-time
- Bst DNA polymerase-based isothermal amplification
- Applicable to POC MDx
Products
| Cat.No. | Product | Size |
|---|---|---|
| FRLMP2-100 | HelixAmp™ Fast RT-LAMP Kit (Ver. 2.0) | 100 rxns |
| FRLMP2-500 | HelixAmp™ Fast RT-LAMP Kit (Ver. 2.0) | 500 rxns |
HelixAmp™ Fast RT-LAMP Kit (Ver. 2.0) provides simple and fast (within 20 minutes) target RNA amplification using loop-mediated isothermal amplification (LAMP). This kit consists of a 5x RT-LAMP Buffer V2 (Mg-free), 100mM MgSO₄ and a RT-LAMP Enzyme V2. The 5x RT-LAMP Buffer V2 (Mg-free), optimized for fast amplification, contains buffering reagents, dNTPs, and salts. The RT-LAMP Enzyme V2 is a blend of engineered Bst DNA polymerase, thermostable reverse transcriptase (RTase), and RNase inhibitor. The novel Bst DNA polymerase enhances the DNA polymerization speed and allows the fast isothermal amplification reaction. The thermostable RTase is fully active at a relatively high temperature (60 ℃) and it makes possible the one-step RT-LAMP in a constant reaction temperature.
