DNA-RNA Amplification Isothermal Amplification
Fast RT-LAMP Kit (Ver.3.0)
- The fastest one-step RNA amplification: Eight minutes to threshold
- Unparalleled sensitivity: As few as 10 to 50 copies of the target RNA in a reaction
- Unmatched specificity: Guarantee clear differentiation from background amplifications
- Ensuring accurate and reliable results: Experience next-level isothermal amplifications
Products
| Cat.No. | Product | Size |
|---|---|---|
| FRLMP3-100 | HelixAmp™ Fast RT-LAMP Kit (Ver. 3.0) | 100 rxns |
| FRLMP3-500 | HelixAmp™ Fast RT-LAMP Kit (Ver. 3.0) | 500 rxns |
HelixAmp™ Fast RT-LAMP Kit (Ver. 3.0) provides simple and fast (within 20 minutes) amplification of target RNA/DNA through loop-mediated isothermal amplification (LAMP). Especially, this kit effectively mitigates non-specific product formation during isothermal amplification. The components include a 5x RT-LAMP Buffer V3 (Mg-free), 100mM MgSO₄ and a RT-LAMP E.M V3. The 5x RT-LAMP Buffer V3, optimized for fast amplification, comprises buffering reagents, dNTPs, and salts. The RT-LAMP E.M V3 is a blend of engineered Bst DNA polymerase, thermostable reverse transcriptase (RTase), and RNase inhibitor. The novel Bst DNA polymerase enhances the DNA polymerization speed and allows the fast isothermal amplification reaction. The thermostable RTase maintains full activity at a relatively high temperature (60℃), enabling one-step RT-LAMP in a constant temperature.
