HelixAmp™ Genome Amplifier (WGA) is suitable to amplify the whole genome from very little amount of genomic DNA. The genome amplification is performed by phi29 DNA polymerase with an isothermal MDA (multiple displacement amplification)–based mode. Because of the strand displacement activity, strong 3’→5’ exonuclease activity and extreme processivity, phi29 DNA polymerase could produce DNA up to 100 kb long with high fidelity. About 1,000 fold amplified DNA can be obtained from 10 ng genomic DNA within 90 min using this kit. The amplified genome can be directly applied to downstream genetic analysis works including PCR, genotyping, and library constructions.

 

 

 

Figure 1. WGA reactions from 10 ng of human genomic DNA using HelixAmp™ Genome Amplifier (WGA).

Reactions were performed in 20 μl volume at 30 ℃ for 1.5 hours. 3 μl of each reaction solution was loaded on a 1% agarose gel for analysis. 

 

 

 

 

 

Figure 2. Time-course measurements of DNA amplification using HelixAmp™ Genome Amplifier (WGA) from 10 ng of genomic DNA purified from blood collections.

10 ng of genomic DNA samples were amplified using the HelixAmp™ Genome Amplifier (WGA), and the DNA yields of each reaction were determined by reaction time. About 8 μl of products were obtained in 60 minutes and 12 μl after 90 min incubations. 

 

 

 

 

 

Figure 3. Validation of amplified genome products by chromosome-targeting PCR analysis.

10 ng of human genomic DNA, isolated from blood collection, was applied in the whole genome amplification using HelixAmp™ Genome Amplifier (WGA). (A) PCR detections of human beta-actin gene from the amplified genomes. Purification is not required for the PCRs using the products of HelixAmp™ Genome Amplifier (WGA). (B) PCR detections of each chromosome-specific region of chromosome 7, 8, 10, and 17 were performed using the amplified whole genome as the template. The amounts of the template used in each 50 μl PCR reactions are: lane 1, 1 μl; lane 2; 0.1 μl, lane 3; 0.01 μl of the amplified genome product and lane 4; 1 ng of the purified human genomic DNA as a control.

Storage

• -20 ± 5 ℃

Shelf life

• 6 months