PureHelix™ Fast-n-Pure Plasmid Kit (Ver. 2.0) is a simplified and fast kit for isolation of pure plasmids up to 20 μg for routine molecular biological applications, such as PCR, cloning, sequencing, in vitro transcription, transfection experiments, etc. PureHelix™ Fast-n-Pure Plasmid Kit (Ver. 2.0) is based on alkaline-lysis method and the binding properties of DNA on silica membrane to provide the high-quality and high-yield plasmid DNAs. The applied pH indicating system in this kit helps to visual verification of purification process and prevent handling errors. PureHelix™ Fast-n-Pure Plasmid Kit is designed that its whole process could be completed within 10 min, while other conventional products require at least 25 min to purify the plasmid using mini-columns.

 

 

Figure 1. An isolation of large size plasmids using PureHelix™ Fast-n-Pure Plasmid Kit (Ver. 2.0). 

 

 

 

 

 

Figure 2. A purification of high-copy and low-copy plasmid DNAs using PureHelix™ Fast-n-Pure Plasmid Kit (Ver. 2.0). 

 

 

 

 

 

Figure 3. Restriction enzyme digestion of the purified plasmid using PureHelix™ Fast-n-Pure Plasmid Kit (Ver. 2.0). 

Restriction enzymatic analysis of pUC19 DNA purified using PureHelix™ Fast-n-Pure Plasmid Kit (Ver. 2.0) 1 : Isolated uncut pUC19, 2 : Digested pUC19 with BamHI, 2 : Digested pUC19 with TaqI, 3 : Digested pUC19 with XbaI. 

 

 

 

 

 

Figure 4. The sequence analysis for plasmid DNA isolated by PureHelix™ Fast-n-Pure Plasmid Kit (Ver. 2.0).

Storage

• Room temperature
  

 

Shelf life

• 12 months