This is the bulk version of HelixAmp™ Taq Polymerase.
If you would like to purchase in bulk, please contact sale@nanohelix.net.
HelixAmp™ Taq Polymerase is a recombinant enzyme expressed and purified from a bacterial host cell harboring Thermus aquaticus DNA polymerase gene. HelixAmp™ Taq Polymerase is an engineered Taq DNA polymerase enforced its thermostability and template sensitivity. This highly purified thermostable DNA polymerase with unique NanoHelix’s purification process is quite suitable for routine PCR.
The separately available 10x reaction buffer contains pH-buffering agents, salts, Mg2+, and dNTPs.
Application
- Routine PCR and RT-PCR
- Conventional and Real-Time PCR
- PCR for molecular diagnostics
- Manufacture of amplification mixtures
Figure 1. The purity of HelixAmp™ Taq Polymerase.
The purity and concentration of the prepared enzyme were analyzed by SDS-PAGE with each unit represented. St: Standard Protein marker.
Figure 2. Comparison of the PCR performances of commercial Taq polymerases on various sizes of targets.
The PCRs were performed with 1.25 units of Taq polymerases and supplied buffers from each manufacturer. All other components and conditions applied are identical throughout the reactions.
Figure 3. The sensitivity of HelixAmp™ Taq Polymerase.
HelixAmp™ Taq Polymerase amplified targets from the genomic region of human PKD (polycystic kidney disease) gene at various concentrations of human genomic DNA. 1.25 U of Taq DNA polymerases manufactured by NanoHelix and other companies were used.
Figure 4. Superior activity of HelixAmp™ Taq Polymerase.
The PCRs were performed with indicated units of Taq polymerases and supplied buffers from each manufacturer. All other components and conditions applied are identical throughout the reactions. NC, negative control reactions were performed with 2.5 units of enzymes without template DNA.
Enzyme activities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
Temperature optimum: Approximately +75 ℃
Storage buffer: 20 mM Tris-HCl (pH 9.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers and 50 % (v/v) glycerol
Purity: ≥ 95 % (SDS-PAGE)
DNase contamination test: Not detectable (Incubation with 40 U enzyme and pUC19 plasmid at 37 ℃, 1 hr)
RNase contamination test: Not detectable (Incubation with 40 U enzyme and human total RNA at 37 ℃, 1 hr)
DNA contamination test: [E.coli DNA] less than 1 copy/5 U enzyme, [Human DNA] Not detectable
Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)
Stability: 24 months at -20 ℃
