This is the bulk version of RealHelix™ qPCR Kit [Probe].

If you would like to purchase in bulk, please contact sale@nanohelix.net.

RealHelix™ 2x qPCR Premix [Probe] is designed to perform a rapid real-time quantification of target DNA using dual-labeled probes. The convenient 2x concentrated premix contains hot-start PCR enzyme, dNTPs, buffers, Mg²⁺, and stabilizing agent. The hot-start PCR enzyme provides high specific amplification of target DNA and minimizes the non-specific reactions and production of primer dimers. The separately supplied ROX dye could be used as a passive reference dye in real-time PCR instruments that are compatible with the evaluation of the ROX signal. Bulk version is premix only. ROX reference dye is available separately upon request.

Application

• Real-time quantification of multiple target DNAs
• Automated high throughput real-time PCR
• Gene expression analysis of cDNA
• SNP analysis and genotyping
• Microbial & viral pathogen detection
• Molecular diagnosis

Figure 1. The accuracy of real-time PCR using RealHelix™ qPCR Kit [Probe].

Human genomic DNAs (10, 1, 0.1, and 0.01 ng) isolated from whole blood were analyzed using the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene-specific primer set and 5’-FAM-labelled probe. The accuracy of real-time PCR is demonstrated by E=99.9 %, R² value=0.997. Performed on a CFX96 real-time PCR detection system (Bio-Rad).

Figure 2. Comparison of real-time PCR using RealHelix™ qPCR Kit [Probe] and its previous version (qPCR Kit [Probe type]).

Human genomic DNAs (100, 10, 1, and 0.1 ng) isolated from whole blood were analyzed using the beta-globin gene-specific primer set and 5’-Quasar705-labelled probe. Performed on a CFX96 real-time PCR detection system (Bio-Rad).

Properties: Real-time PCR, probe type, hot-start

Heat activation: +95 ℃ for 15 minutes

Activity test: Performance test in PCR using Bio-Rad CFX-96 (Human genomic DNA, β-globin fragment): Corresponds to specification